敲除MIG1同时过表达MAL62面包酵母的发酵性能

摘要

The fermentation abilities of the recombined strain BPM-M and its overexpressing MAL62 along with deletion of MIG1 were investigated. Our results showed that the recombined strain BPM-M has the following advantages over the paren-tal strain BY-14a. Maltose utilization was 29.1%higher,glucose repression of BPM-M was reduced by 38.8%,and the leav-ening ability of the lean dough was enhanced to 163.4,mL/(h·g),which was 50.6%higher than that of BY-14a.%以亲本面包酵母工业菌株 BY-14a 为对照，研究了敲除 MIG1同时过表达 MAL62面包酵母重组菌 BPM-M的发酵性能．实验结果表明：重组菌株 BPM-M 的麦芽糖酶活提高，麦芽糖利用速率较亲本菌株提高29.1%；葡萄糖阻遏减弱38.8%；无糖面团发酵力达到163.4,mL/(h·g)，较亲本菌株提高了50.6%．