清热消炎颗粒解热作用及对血清中TNF-α、IL-1β、IL-6和PGE2影响的初步研究

摘要

目的：观察清热消炎颗粒对内毒素致发热大耳白兔的解热作用及对血清中TNF-α、IL-1β、IL-6、前列腺素（PGE2)表达水平的影响，探讨清热消炎颗粒的解热机制。方法：体温合格的大耳白兔36只随机分为6组，分别为对照组，模型组，阿司匹林组，清热消炎颗粒低、中、高剂量组。一次灌胃给予药物（阿司匹林、清热消炎颗粒）后大耳白兔耳缘静脉注射内毒素溶液制备发热模型。监测给药前、内毒素注射后1、2、3、4 h体温。内毒素注射4 h后，测定血清TNF-α、IL-1β、IL-6、PGE2水平。结果：清热消炎颗粒中、高剂量组，在内毒素注射后1、2、3、4 h直肠温度升高值均显著低于模型组（P＜0．05或P＜0．01）。而低剂量组与模型组比较，直肠温度无显著性差异。内毒素致热后4 h，模型组大耳白兔血清TNF－α、IL－1β、IL－6、PGE2水平较对照组显著升高（P＜0．01）；与模型组比较，清热消炎颗粒高剂量组TNF-α、IL-1β、IL-6、PGE2水平均显著降低（P＜0.05）。结论：中、高剂量清热消炎颗粒有明显解热作用，低剂量药物无显著降温作用。清热消炎颗粒高剂量组可明显降低内毒素致热大耳白兔血液的内生致热原TNF-α、IL-1β、IL-6及PGE2。%O bjective:To observe the antipyretic effect of Qingrexiaoyan particles on the pyrexial rabbits induced by endotoxin ,and the influence on expression levels of TNF-α, IL-1β, IL-6, PGE2 and further explore the antipyretic mechanism of Qingrexiaoyan particles. Methods:Thirty six rabbits with normal temperature were randomly divided into six groups:control group, model group, aspirin group, Qingrexiaoyan particles low dose group, middle dose group and high dose group. The fever models were induced by injecting endotoxin into the ear vein of rabbits after a lavage for drugs. Temperature was monitored separately before the administration and after the endotoxin injection 1,2,3,4 h. After endotoxin injection 4 h, the serum TNF-α, IL-1β, IL-6, PGE2 levels were assayed. Results:Rectal temperature of Qingrexiaoyan particles middle dose group and high dose group increased continuously after the endotoxin injection, 1,2,3,4 h after injection, rectal temperature were significantly lower than the model group (P<0.05或P<0.01). No significant difference was found between low-dose group and model group. After endotoxin injection 4 h, the serum TNF-α, IL-1β, IL-6, PGE2 levels of model group were higher than the control group (P<0.01);compared with the model group, the TNF-α, IL-1β, IL-6, PGE2 levels of high Qingrexiaoyanning particles dose group were significantly lower (P<0.05). Conclusion:The results show that Qingrexiaoyan particles middle dose group and high dose group have marked antipyretic effect but the low dose group has no such effect. The high-dose group of Qingrexiaoyan particles can significantly reduce the endogenous pyrogen of endotoxin-induced fever rabbits, such as TNF-α, IL-1β, IL-6 and PGE2.