六堡茶对胰岛素抵抗3T3-L1脂肪细胞糖脂代谢的影响

摘要

To investigate the effect of Liupao tea on glucose and lipid metabolism in palmitate-induced insulin resistance 3T3-L1 adipocytes. Glucose intake decreased and insulin resistance was induced in 3T3-L1 adipocytes after incubation in 1mmol/L palmitate for 24 hours. Compared with the model group, the concentrations of glucose and NEFA decreased along with the increase of Liupao tea concentrations in medium. The mRNA expression of key enzyme for glycolysis of glucose (Hexokinase, 6-phosphofructokinase-1, and pyruvatekinase) and the Lipid-Metabolism-Related Enzymes (Sn-glycerol-3-phosphate acyltransferase) significantly increased in the Liupao tea groups comparing with model sample. Meanwhile the glucose transporter 4 mRNA expression was also increased and the acetyl-CoA carboxylase, protein tyrosine phosphatase 1B mRNA expression was decreased. Low doses of Liupao tea reduced carnitine palmitoyl transferase I mRNA expression while high doses significantly raised it. Research proved that Liupao tea were able to increase glucose uptake and activate the glucolipid metabolic pathways.Liupao tea showed the effect of improving insulin resistance induced by palmitate in 3T3 -L1 adipocytes.%采用软脂酸诱导3T3-L1脂肪细胞胰岛素抵抗后，给予不同剂量的六堡茶水提物治疗，探讨六堡茶对胰岛素抵抗3T3-L1脂肪细胞的糖脂代谢的影响。结果表明，1 mmol·L-1的软脂酸对3T3-L1脂肪细胞作用24 h后，葡萄糖摄取量减少，建立胰岛素抵抗模型。六堡茶对模型细胞干预24 h 后，与模型组比较，细胞培养基的残留液中葡萄糖和游离脂肪酸的含量降低；对糖脂代谢相关关键酶（己糖激酶、6-磷酸果糖激酶-1、丙酮酸激酶、甘油-3-磷酸酰基转移酶）和葡萄糖转运子基因表达都有上调作用，并下调乙酰辅酶 A 羧化酶、蛋白酪氨酸磷酸酯酶1B基因表达。低剂量的六堡茶下调肉碱脂酰转移酶-I基因的表达，但是高剂量能显著上调肉碱脂酰转移酶-I基因表达。实验证明了六堡茶能够提高胰岛素抵抗3T3-L1脂肪细胞对葡萄糖的摄取能力，同时促进细胞内糖脂代谢，六堡茶有改善3T3-L1脂肪细胞胰岛素抵抗的作用。