Inhibition of Calcium-Independent Phospholipases A2beta or A2gamma Inhibit Hormone-Induced Differentiation of 3T3-L1 Preadipocytes.

摘要

A method for identifying an agonist exhibiting molecular or pharmacologic inhibition which is effective against the activity of at least one of iPLA(sub 2)beta and iPLA(sub 2)gamma which comprises culturing 3T3-L1 cells and transfecting them with negative control siRNA, siRNA directed against iPLA(sub 2)beta or siring directed against iPLA(sub 2)gamma prior to induction or during to differentiation or pharmacologic inhibition and observing for whether that down regulation of iPLA(sub 2)beta or iPLA(sub 2)gamma inhibits adipocyte differentiation.