Inhibition of calcium-independent phospholipases A2beta or A2gamma inhibit hormone-induced differentiation of 3T3-L1 preadipocytes

摘要

A method for identifying an agonist exhibiting molecular or pharmacologic inhibition which is effective against the activity of at least one of iPLA₂β and iPLA₂γ which comprises culturing 3T3-L1 cells and transfecting them with negative control siRNA, siRNA directed against iPLA₂β or siring directed against iPLA₂γ prior to induction or during to differentiation or pharmacologic inhibition and observing for whether that down regulation of iPLA₂β or iPLA₂γ inhibits adipocyte differentiation.