四川、重庆不同紫苏遗传多样性的SRAP研究

摘要

目的:研究四川重庆不同紫苏材料的遗传多样性,为种质资源的分类和选育打下理论基础.方法:采用SRAP分子标记方法对来自四川、重庆的10份紫苏材料进行遗传多样性分析,利用popgene1.32及NTSYS_pc2.1软件处理数据,UPGMA方法聚类,构建亲缘关系树状图.结果:26对SRAP引物组合扩增出167条带,多态比率(PPB)为51.57％.10份材料的遗传距离变化范围为0.098 8～0.916 3,平均为0.490 8.用籽材料中,四川和重庆地区的平均多态位点百分率为27.25％,在两个地区间,SRAP标记的平均H值分别为0.149 6和0.232 8.平均Ⅰ值为0.221 1和0.350 8.结论:SRAP分子标记适合用于紫苏材料的遗传多样性分析,能够清晰地揭示不同材料间的亲缘关系,为下一步紫苏的良种选育提供科学依据.%Objective of the study: The genetic diversity of Perilla frutescen (Linn). Britt in Sichuan and Chongqing has been researched in this paper to make Chongqing estimated by means of sequence-related amplified polymorphism (SRAP). The date has been analyzed by the theoretical basis for promising germ-plasm of identification and further breeding. Methods of the study: Ten materials in Sichuan and Popgene 1. 32 and NTSYS-pc2. 1 software. The system diagram of genetic relationship has been built by UPGMA. Results of the study:26 pairs of SRAP primers combination amplified 167 polymorphic bands, and the average percentage of polymorphic bands reaches to 51. 57%. The Nei's genetic identity of 10 samples ranges in 0. 098 8-0. 916 3. The average is 0. 490 8. The average of percentage of polymorphic bands of materials used breeds in Sichuan and Chongqing is 27. 25%. Among the two areas: The average of Nei's gene diversity (H) is 0. 149 6 and 0. 232 8 by SRAP marker. The average of Shannon's information index (I) is 0. 221 1 and 0. 350 8 by SRAPs. Conclusion of the study: SRAP markers can be appropriately applied to genetic analysis in Perilla frutescens (Linn). Britt. It can possess several genetic relationships syllabified to provide the scientific basis.