重庆市缙云山部分蕨类植物的SRAP分子标记与分类

摘要

[Objective] The aim was to classify pteridophytes by using SRAP molecular marker.[Method] 27 species of pteridophytes collected from Jinyun Mountain in Chongqing were classified based on SRAP molecular marker.The tender leaves of the 27 species of city pteridophytes were use for extracting genonic DNA of pteridophytes by using improved CPAB method,and then the quality of total DNA was tested by using gel-lectro phoresis method and obsorbance method.[Result] The results of clustering analysis showed that there was close genetic relationship between Selaginellaceae and Gleicheniaceae,Equisaceae and Dryopteridaceae,Blechnaceae and Dryopteridaceae,Lindsaeaceae and Dryopteridaceae,Aspleniaceae and Athyriaceae.Close genetic relationship between Cyrtomium fortunei and Dryopteridaceae differed from traditional taxonomy that classified Cyrtomium fortunei in Dryopteridaceae,indicating that classification of pteridophytes by SRAP molecular marker technology had some differences from traditional taxonomy.[Conclusion] The results can be referred to rebuild phylogenetic relationships of pteridophytes.%[目的]利用SRAP分子标记的方法对蕨类植物进行种属分类。[方法]以采集于重庆市缙云山的27种蕨类植物为研究对象,以其嫩叶为材料用改良后的CTAB法提取蕨类植物的基因组DNA,用凝胶电泳和吸光值法对总DNA的质量进行检测,用SRAP分子标记技术对这27种蕨类植物进行了分类研究。[结果]27种蕨类植物的SRAP标记聚类分析图谱表明,卷柏科与里白科、木贼科与鳞毛蕨科、乌毛蕨科与鳞毛蕨科、铁角蕨科与蹄盖蕨科、鳞始蕨科与鳞毛蕨科的亲缘关系比较接近;试验结果与传统分类学中贯众属属于鳞毛蕨科有冲突。[结论]用SRAP分子标记的方法得到的结果与传统分类结果存在一定的差异,但这种差异很小,试验结果可以为重建蕨类植物的系统演化关系提供参考。