目的 探讨IL28B基因单核苷酸多态性位点(SNP)rs12979860和rS8099917与e抗原阳性慢性乙型病毒性肝炎病人 对干扰素治疗应答反应性的关系.方法 采集入组的82例应用干扰素治疗的病人的外周血(38例对干扰素应答,44例对干扰素不应答).提取全基因组DNA,并扩增IL28B基因.SNPrs8099917用聚合酶链式反应-限制性片段长度多态性法分型,SNPrs12979860直接测序分型.对分型结果进行统计学分析.结果 在干扰素治疗应答组,SNPrs8099917基因型TT,TG+GG的比例分别是81.6％(31/38)和18.4％(7/38),等位基因G的基因频率是9.2％(7/76),在干扰素治疗非应答组,SNPrs8099917基因型TT,TG+GG的比例分别是97.7％(43/44),2.3％(1/44),等位基因G的基因频率是1.1％(1/88),两组间的差异有统计学差异(基因型P=0.014；基因频率P=0.025).SNPrs12979860基因型CT的比例和等位基因T频率在干扰素治疗应答组和非应答组均无显著差异(P＞0.05).结论 SNPrs8099917可能与e抗原阳性慢乙肝病人对干扰素治疗应答反应性存在相关性,等位基因G可能预示着干扰素治疗的成功应答,该位点基因型检测对于接受干扰素治疗的慢性HBV感染者可能有一定的疗效预测价值.%Objective To explore the relationship between the single nucleotide polymorphisms (SNPs) of rs12979860 and rs8099917 in IL28B gene and the response to interferon treatment in hepatitis B e antigen (HBeAg)-positive patients with chronic hepatitis B patients. Methods Peripheral blood samples were collected from 82 HBeAg-positive patients with chronic hepatitis B receiving interferon treatment, including 38 with favorable response to the treatment and 44 without response. IL28B gene was amplified from the chromosomal DNA, and rs8099917 SNP was genotyped based on PCR-RLFP and rsl2979860 SNP by sequencing. Results In the responsive patients, the distribution frequencies of TT and TG+GG genotypes and allele G in SNPrs8099917 were 81.6% (31/38), 18.4% (7/38), and 9.2% (7/ 76), as compared to the frequencies of 97.7% (43/44), 2.3% (1/44), and 1.1% (1/88) in nonresponsive patients, respectively. The frequencies showed significant differences between the responsive and nonresponsive patients (P= 0.014 for genotypes and P=0.025 for allele G). The distribution frequencies of CT genotypes and allele T in SNPrsl2979860 showed no differences between the responsive and nonresponsive patients (P0.05). Conclusion rs8099917 SNP is probably associated with the response to interferon treatment in HBeAg-positive patients with chronic hepatitis B, and Allele G may be predictive of the treatment success.
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