miR-145在糖尿病性勃起功能障碍大鼠发病机制中的作用

摘要

Objective To investigate the role of miR-145 in the corpus cavernosum smooth muscle tissue in the pathogenesis of erectile dysfunction (ED) in diabetic rats. Methods The total RNA was extracted from the corpus cavernosum of a diabetic rat model with ED, diabetic rats with normal erectile function and normal rats, and the expression levels of miR145 were compared between the groups. Results The expession of miR-145 was decreased in the corpus cavernosum of diabetic rats with ED. Conclusion Diabetes mellitus can cause ED in rats, in which process decreased expression of miR145 in the corpus cavernosum may play a role.%目的 探讨miR-145基因在糖尿病性勃起功能障碍(ED)大鼠发病过程中的作用.方法 建立糖尿病性ED大鼠动物模型,取阴茎海绵体组织,提取总RNA,qRT-PCR法检验miR-145在糖尿病性ED大鼠、糖尿病性勃起功能正常大鼠及正常大鼠阴茎海绵体组织中表达差异.结果 miR-145在糖尿病性ED大鼠阴茎海绵体组织中的表达量下降.结论 糖尿病可导致大鼠阴茎ED,miR-145基因表达下调可能与ED发生有关.