目的：探讨姜黄素体外诱导人T细胞淋巴瘤Jurkat细胞凋亡时MAPKs及MMPs家族成员的表达，揭示其可能的分子机制。方法以不同浓度姜黄素作用Jurkat细胞不同时间，MTT检测细胞增殖抑制率，流式细胞术检测细胞周期变化，Western-blot检测MAPKs家族成员的表达，明胶酶谱法检测细胞培养上清中MMPs的活性。结果姜黄素呈时间-剂量依赖性抑制Jurkat细胞增殖，使细胞阻滞于G0/G1期。以6.25、12.50及25.00μmol/L姜黄素分别处理Jurkat细胞，胞内JNK和P-JNK的表达均呈浓度依赖性上升（P<0.01），而ERK1/2及P38 MAPK的表达与阴性对照组相比无明显改变。另细胞培养上清中MMP-2及MMP-9的活性在各药物处理组中也基本不变。结论姜黄素在（6.25~25.00）μmol/L浓度范围时，可体外诱导Jurkat细胞的凋亡，阻滞细胞于G0/G1期。其分子机制可能与激活MAPKs家族中的JNk信号通路有关，而与MMPs家族成员的活性无关。%Objective To investigate the expressions of mitogen- activated protein kinases (MAPKs) and matrix metalloproteinases (MMPs) in apoptotic human T cell lymphoma Jurkat cells induced by curcumin in vitro and explore the possible molecular mechanisms of curcumin- induced apoptosis. Methods Jurkat cells were treated with different concentrations of curcumin, and the cell proliferation and cell cycle changes were detected by MTT assay and flow cytometry, respectively. Western blotting and gelatin zymography were employed to examine the protein expression levels of MAPKs and MMPs activity in the exposed cells. Results Curcumin inhibited the proliferation of Jurkat cells in a time-and dose-dependent manner, and caused cell cycle arrest in G0/G1 phase. Treatment of Jurkat cells with 25, 50, and 75μmol/L curcumin resulted in a concentration-dependent increase of JNK and p-JNK expressions (P<0.01) without significantly affecting the expressions of ERK1/2 and P38 MAPK or the activity of MMP-2 and MMP-9. Conclusion Curcumin within the concentration range of 6.25-25.00μmol/L can induce apoptosis and cell cycle arrest of Jurkat cells, the mechanism of which might involve the activation of JNK pathway but not the MMPs.
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