肝细胞癌中GPC3和Notch1表达的关系

摘要

Objective To investigate the correlation between the expressions of glypican-3 (GPC3) and Notchl and their roles in the tumorigenesis and progression of hepatocellular carcinoma (HCC). Methods Immunohistochemistry and computerized image analysis were utilized to quantitatively detect the expressions of GPC3 and Notchl in 30 HCC tissue specimens. Results In the 30 HCC specimens, GPC3 expression decreased significantly as the grade of tumor differentiation increased (PO.05 or P0.01), while Notchl expression presented with a reverse pattern of changes (P0.05 or P<0.01). An obvious negative correlation was found between the expressions of GPC3 and Notchl in HCC tissues (rp=-0.607, P=0.000; r=-0.692, P=0.000). Conclusion The expressions of GPC3 and Notchl show a negative correlation in HCC, suggesting a possible mechanism for mutual regulation between them to contribute to the tumorigenesis and progression of HCC.%目的 通过观察肝细胞癌组织中磷脂酰肌醇蛋白聚糖3(GPC3)与Notch1的表达情况,探讨两者在肝细胞癌中表达的相互关系.方法 应用免疫组化方法、计算机图像分析等技术检测30例肝细胞癌组织中GPC3和Notch1的表达情况,并用SPSS软件系统进行统计学分析.结果 肝细胞癌组织分化程度越高,GPC3表达越低；分化程度越低,GPC3表达越高,差异有显著性(P＜0.05)或高度显著性(P＜0.01).肝细胞癌组织化程度越高,Notch1表越高；分化程度越低,Notch1表达越低差异有显著性(P＜0.05)或高度显著性(P＜0.01).GPC3与Notch1的表达呈高度显著性负相关(rp=-0.607,P=0.000; r=-0.692,P=0.000).结论 在肝细胞癌中,GPC3与Notch1的表达呈现负相关性,提示两者间可能存在相互调节的机制,共同影响肝细胞癌的发生发展.