多胺及其合成抑制剂对干旱胁迫下杏苗生理指标的影响

摘要

[Objective]The present experiment was conducted to study effects of polyamine and its synthetic inhibitor methylglyoxal bis-guanylhydrazone(MGBG) on physiological indexes of Armeniaca vulgaris Lam. Jintaiyang seedlings un-der drought stress, in order to provide reference basis for anti-drought cultivation. [Method]One-year grafted seedlings of A. vulgaris Lam. Jintaiyang were taken as experimental materials. Six treatments including treatment 1(CK),treatment 2(D.), treatment 3[CK+spermine(Spd)],treatment 4(D.+Spd),treatment 5(D.+MGBG) and treatment 6(D.+MGBG+Spd) were applied. The seedlings were sprayed one time every two days. Contents of malondialdehyde(MDA),soluble sugar and pro-line(Pro) and chlorophyll, photosynthetic parameters in leaves of A. vulgaris Lam. Jintaiyang seedlings were measured af-ter being treated for 30 days. [Result]Under drought stress, contents of MDA,soluble sugar and Pro in A. vulgaris Lam. Jintaiyang seedling leaves increased, and total content of chlorophyll and photosynthetic parameters decreased. Spd (1.0 mmol/L) treatment improved contents of MDA,soluble sugar and Pro, total content of chlorophyll and most photosynthetic parameters in A. vulgaris Lam. Jintaiyang seedling leaves. Spd(1.0 mmol/L) treatment under drought stress increased sig-nificantly contents of MDA, soluble sugar and Pro, total content of chlorophyll and most photosynthetic parameters in A. vulgaris Lam. Jintaiyang seedling leaves(P<0.05). MGBG(1.0 mmol/L) treatment under drought stress decreased contents of MDA,soluble sugar and proline,total content of chlorophyll and most photosynthetic parameters in A. vulgaris Lam. Jin-taiyang seedling leaves. [Conclusion]Spd(1.0 mmol/L) treatment can improve A. vulgaris Lam. Jintaiyang seedlings adap-tation capacity to drought stress, which can be applied in large-scale cultivation.%【目的】探讨喷施多胺及其合成抑制剂甲基乙二醛双脒基腙（MGBG）对干旱胁迫杏苗生理指标的影响，为杏抗旱栽培提供参考依据。【方法】以金太阳杏1年生嫁接苗为试材，设处理1（对照，CK）、处理2[干旱（Drought， D.）胁迫]、处理3[（CK+亚精胺（Spermidine，Spd）]、处理4（D.+Spd）、处理5（D.+MGBG）和处理6（D.+MGBG+ Spd）共6个处理，每2 d处理1次，处理30 d后分别测定并分析各处理叶片丙二醛（MDA）、可溶性糖、游离脯氨酸（Pro）含量、叶绿素总含量及光合参数。【结果】干旱胁迫下，杏苗叶片MDA、可溶性糖和Pro含量增加，叶绿素总含量和光合参数均降低；喷施1.0 mmol/L Spd的杏苗叶片MDA含量、可溶性糖含量、Pro含量、叶绿素总含量及大部分光合参数提高；在干旱胁迫下喷施1.0 mmol/L Spd可使杏苗的MDA含量、可溶性糖含量、Pro含量、叶绿素总含量及大部分光合参数显著提高（P<0.05）；在干旱胁迫下喷施1.0 mmol/L MGBG可促进杏苗叶片MDA含量、Pro含量、叶绿素总含量及大部分光合参数降低。【结论】喷施1.0 mmol/L Spd可增强金太阳杏苗对干旱胁迫环境的适应能力，可在杏规模化栽培中应用。