蛋白激酶C在肺缺血预处理保护中的作用

摘要

Objective : To investigate the effect of lamotrigine ( LTG ) on learning and memory and the content of NMDA receptor activity in hippocampus of pentylenetetrazol( PTZ )-induced epileptic rats. Methods: Eighty healthy male SD rats , sixteen were assigned randomly control group , sixty four status epilepticus rats were randomly and equally divided into model group , low-dose group , middle-dose group and high-dose group , LTG were intraperitoneally injected every day for two weeks. The ability of learning and memory was tested by Morris water maze and Y maze. The expression of NMDA receptor in hippocampus was determined by immunohistochemical staining. Results : Learning and memory dysfunction was observed in epileptic rats, relative absorbance of NMDA receptor decreased significantly; model group rats treated with low-dose LTG showed no difference in learning and memory function compared with epileptic rats without LTG treatment , relative absorbance of NMDA receptor had no difference ; middle and high-dose LTG appeared to improve the learning and memory function of epileptic rats, relative absorbance of NMDA receptor increased significantly. Conclusion: Status epilepticus can result in damages of learning and memory in immature rats, middle and high-dose LTG appears to iruprove the learning and memory function of epileptic rats, which might be relevant to its regulating effect on the expression of NMDA receptor in hippocampus.%目的:建立大鼠肺缺血/再灌注(I/R)损伤模型,探讨蛋白激酶C(PKC)在肺缺血预处理(IPC)保护中的作用.方法:建立在体单侧肺原位I/R模型.50只SD大鼠随机均分为假手术(S)组、I/R组、IPC组、多黏菌素B(PMB)组和缺血预处理加多粘菌素B(IPC+PMB)组5组.实验结束时测定各组肺组织SOD活力、MDA含量、肺湿干重比和肺泡损伤数比值,观察肺超微结构变化.结果:(1) 与S组相比,I/R组SOD活力下降,MDA含量升高,肺湿干重比升高,肺泡损伤数比值升高(均P＜0.01);与I/R组比较,IPC组SOD活力升高,MDA含量降低,肺湿干重比降低,肺泡损伤数比值降低(均P＜0.01);与I/R组比较,PMB组和IPC+PMB组各项指标差异无统计学意义(均P＞0.05).(2) 肺组织病理学改变:I/R组、PMB组和IPC+PMB组肺组织损伤明显,IPC组肺组织损伤较I/R组明显减轻,S组肺组织结构基本正常.结论:IPC对肺I/R损伤有明显的保护作用,而且这一作用可被PKC抑制剂PMB抑制.