猪伪狂犬病病毒新流行株主要糖蛋白的分子特征分析

摘要

Objective] In order to study the genetic characteristics of the novel epidemic pseudorabies vi-rus ( PRV) .[Method]A PRV strain named PRV AH was isolated from a pig farm in Anhui Province , the phylogenetic tree was constructed and the glycoproteins gB ,gC,gD and gE of this strain were analyzed .[Result] PRV AH was very similar to an epidemic strain which had caused a severe PRV outbreak in China since 2011 , but was distantly related to Bartha or other foreign strains .Compared with Bartha and other foreign strains , all PRV strains in China had obvious deletions and insertions of continuous amino acids in the glycoproteins , and some mutations were found in the critical functional regions of the glyco-proteins .[Conclusion ]This study analyzes the genetic differences between the novel epidemic PRV strain and Bartha strain at the molecular level , which provides a theoretical basis for further clarification of the immunization failure of Bartha-K61 vaccine .%【目的】进一步了解猪伪狂犬病毒新流行株的遗传特征。【方法】对从安徽某猪场分离的1株猪伪狂犬病病毒（ PRV） AH株进行遗传进化分析及主要糖蛋白gB、gC、gD、gE的序列分析。【结果】PRV AH株与国内2011年以来的流行毒株非常相似，但与Bartha株及其他国外毒株遗传关系较远；国内流行毒株与Bartha株及其他国外毒株相比，其主要糖蛋白均存在明显的连续氨基酸的缺失和插入，且部分突变位点位于这些糖蛋白的重要功能区。【结论】从分子水平上解析了PRV新流行株与疫苗株Bartha株的遗传差异，为进一步深入研究Bartha株疫苗免疫失败的原因提供了理论依据。