几种植物精油对红阳猕猴桃中两种致病菌抑制效果的研究

摘要

Obj ective]The aim of the study was to investigate inhibitory effects of plant essential oil on pathogens in the'Red Sun'kiwifruit.In addition,the plant essential oil that had good fresh-keeping effect was selected.[Method]In this study,the dilution method was used to select the essential oils which had inhibitory effects against two pathogens of'Red Sun'kiwifruit.Moreover, the minimal inhibitory concentration (MIC)and minimum bactericidal concentration (MBC)of es-sential oils were determined.[Results]The minimal inhibitory concentration of cinnamon essen-tial oil,thyme oil and clove oil against Colletotrichumacutatum were 0. 125×104 ,0. 500×104 and 1. 00×104μL/L,respectively.The minimum bactericidal concentrations were 0. 125 ×104 ,1. 25 ×104 and 1. 00×104μL/L,respectively.The minimal inhibitory concentrations of cinnamon and peppermint essential oils against Botryosphaeria parva were 0. 125 ×104 and 2. 50 ×104 μL/L. The minimum bactericidal concentrations were 0. 125 ×104μL/L and 10. 0 ×104μL/L,respec-tively.[Conclusion]Cinnamon essential oil,thyme oil,clove oil had good inhibitory effect a-gainst Colletotrichumacutatum.Cinnamon essential oil had high inhibitory activity against Botry-osphaeria parva but peppermint essential oil was not obvious.%【目的】通过研究植物精油在红阳猕猴桃致病菌上的抑制效果，筛选出对红阳猕猴桃有良好贮藏保鲜效果的植物精油。【方法】采用培养基稀释法，利用体积浓度为10．0×104μL/L （v/v）不同植物精油培养基筛选出对红阳猕猴桃中两种致病菌具有抑制效果的精油，并研究其最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。【结果】肉桂精油、百里香精油、丁香精油对尖孢炭疽菌（Colletorichumacutatum）最低抑菌浓度分别为0．125×104，0．500×104，1．00×104μL/L，最低杀菌浓度分别为0．125×104，1．25×104，1．00×104μL/L；肉桂精油、薄荷精油对葡萄座腔菌（Botryosphaeriaparva）最低抑菌浓度分别为0．125×104，2．50×104μL/L，最低杀菌浓度分别为0．125×104μL/L和＞10．0×104μL/L。【结论】肉桂精油、百里香精油、丁香精油对尖孢炭疽菌（Colletorichumacutatum）有较好抑制作用；肉桂精油对葡萄座腔菌（Botryosphaeria parva）具有良好的抑菌及杀菌作用，而薄荷精油对该菌有一定的抑制作用，但杀菌效果不明显。