The change of morphology of podocytes was compared and the difference of cell traction force of podocytes was measured before and after knockout Pinch-3 DNA in this experiment.It was found that a number of pores appeared on the cells surface and the cells projected area increased by average of 40% after gene knockout,but the cell traction force of podocyte were significantly reduced by average of 40%.This suggests that when the expression of the podocytes Pinch-3 gene is inhibited,some pores will appear on the cells surface,and these pores make the membrane of the cells become laxer,and then the cells projected area become larger.The cell traction force decrease correspondingly by average of 40%,which suggests that the pores on the cell surface strongly affect the cell traction force.%用基因敲除的方法比较了正常肾小球足细胞和Pinch-3蛋白缺失细胞的形态差异和牵引力的差异,发现当Pinch-3蛋白缺失后,细胞表面出现了许多孔隙,同时细胞投影面积平均增加40%;细胞的牵引力则平均减小40%左右,这说明当肾小球足细胞的Pinch-3基因的表达受到抑制以后,细胞膜上产生的孔隙使细胞膜变得松散,进而使得表面积增大.细胞牵引力减小40%,说明细胞膜上的孔隙对细胞牵引力的形成具有较强的影响.
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