Objective To establish lymphatic metastasis models of transplanted human pulmonary adenoearcinoma in nude mice and to compare the effect of injection position on tumor growth and lymphatic metastasis. Methods Twenty BALB/c nude mice were divided equally into four groups. A549 cell was injected subcutaneously into the left back near axillar fossa,the right back near axillar fossa,the left hind limb and the left hind nail pad of nude mice in different groups respectively. Tumor size and lymphatic metastasis were dynamicly observed. Results Human transplanted pulmonary adenocarcinoma nude mice models were successfully established in each group. Tumors appear more early and grow faster(P <0. 05) in the left and right back near axillar fossa groups than the other two groups. Lymph node metastasis rates were 41.7% ,42. 9% ,23. 1% and 21. 4% in left near axillar fossa, right back near axillar fossa, left hind limbs and left nail pad groups , respectively. Injection into the left back near axillar fossa was the most convenient. Conclusion Subcutaneous injection of A549 cell in the left back approach axillar fossa of nude mice has the advantages of convenience,fast tumor growth,and high lymph metastasis rate. It might be an optimal animal model of human transplanted pulmonary adenocarcinoma lymphatic metastasis.%目的 构建人肺腺癌的裸鼠皮下移植瘤模型,探讨不同注射部位对肿瘤生长和淋巴转移的影响.方法 20只BALB/c裸鼠随机分为4组,每组5只.分别于左侧背部近腋窝、右侧背部近腋窝、左侧后肢和左后爪垫皮下注射A549细胞株,建立人肺腺癌移植瘤模型,考察各组裸鼠肿瘤生长和淋巴结转移情况.结果 各组裸鼠成瘤明显,模型构建成功.背部近腋窝种植2组裸鼠肿瘤比后肢和爪垫种植的肿瘤出现时间早,生长速度快(P＜0.05).左侧背部近腋窝、右侧背部近腋窝、左侧后肢和左后爪垫皮下移植瘤的淋巴结转移率分别为41.7％、42.9％、23.1％和21.4％.其中左侧背部近腋窝注射最为方便.结论 裸鼠左侧背部近腋窝皮下注射A549细胞操作方便,肿瘤易生长,淋巴转移率高,是构建人肺腺癌皮下移植瘤淋巴转移模型的优选方案.
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