金龙胶囊对荷人肺腺癌 A549裸鼠皮下移植瘤生长的影响

摘要

Objective To explore the effect of Jin Long Capsule with or without cisplatin ( DDP) on tumor growth of lung adenocarcinoma A549 cell xenografts in nude mice .Methods After a model of human lung cancer established by subcutaneous transplantation of A 549 cells in nude mice, 24 mice were randomly divided into control group (A group), Jin Long Capsule group(B group), DDP group(C group) and combination treatment group(D group).Treatment continued for 4 weeks.Inhibition rates were evaluated, tumor micro-vessel density(MVD) were determined by immune-histochemistry, the mRNA expression of VEGF and apoptosis-associated gene(Bcl-2) were assessed by RT-PCR analyses.Results The tumor growth inhibition rates of Jin Long Capsule treatment groups ( B、D groups ) were 26.94% and 51.61%respectively, and more effect in combination group .The expression of MVD in two groups above-mentioned were 18.83 ±0.75 and 14.17 ± 0.75 respectively.No statistically difference were seen between DDP group and control group .Compared to control group, RT-PCR analyses revealed a decrease in expression of Bcl-2 genes in A549 tumors by Jin Long Capsule treatment groups ( B、D groups ) , and no statistically difference in these two groups .Either group showed no statistically difference in VEGF mRNA expression .Conclusion Jin Long Capsule has inhibitory effect , and has synergetic effect with traditional chemotherapy on tumor growth of lung adencarcinoma A 549 cell xenografts in nude mice through inhibiting angiogenesis , and may enhance the sensitivity of A 549 cells to DDP by inducing cell apoptosis .%目的：探讨金龙胶囊对肺腺癌A549移植瘤生长的抑制作用。方法建立裸鼠移植瘤模型，随机分成阴性对照组（ A组）、单药金龙胶囊组（B组）、单药顺铂组（C组）和联合用药组（D组）4组，连续用药4周。观察肿瘤生长情况，计算抑瘤率；免疫组化法检测移植瘤微血管密度（microvessel density，MVD）；RT-PCR分析各组治疗后VEGF及凋亡基因Bcl-2mRNA表达情况。结果与阴性对照组比较，金龙胶囊治疗组（ B、D组）抑瘤率分别为26.94％、51.61％，联合用药组（ D组）效果最佳。治疗组微血管密度分别为18.83±0.75、14.17±0.75，联合用药组最为显著，而单药顺铂组与对照组在抑瘤率和微血管密度表达上均无差异（ P＞0.05）。金龙胶囊治疗组（B、D组）Bcl-2mRNA表达较对照组有显著差异（P＜0.001），但两组之间无差异（P＝0.369）；四组之间在VEGFmRNA表达上无明显差异（ P＞0.05）。结论金龙胶囊对荷A549裸鼠移植瘤有抑制作用，联合顺铂用药有显著协同作用，其作用机制可能与抑制肿瘤微血管形成、诱导细胞凋亡增加有关。