淫羊藿苷抑制大鼠扩弓致牙根吸收过程中牙周组织 TNF-α的表达

摘要

目的：研究淫羊藿苷对大鼠扩弓致牙根吸收抑制过程中肿瘤坏死因子α（TNF-α）表达的影响。方法：24只雄性Wistar 大鼠，随机分成 A（未扩弓对照组）、B（单纯扩弓组）、C（扩弓合并用药组）3组（n ＝8）。B、C 2组分别在每只大鼠的上颌左右第一磨牙间安装扩弓簧，并以初始50 g 的力值连续扩弓2周，扩弓过程中，C 组大鼠给予2．5 mg／（kg·d）淫羊藿苷灌胃，A、B 2组大鼠用等量安慰剂灌胃。2周后处死所有大鼠。取上颌第一磨牙及其牙周组织制作切片。HE 染色观察牙周组织形态变化；TNF-α免疫组化染色，并进行免疫组化光密度分析。结果：A、B、C 组 TNF-α表达的光吸收值分别为0．0302±0．0017、0．2410±0．0024、0．0886±0．0028（B 组与 A 组或 C 组比较，P ＜0．05）。结论：淫羊藿苷可抑制扩弓引起的牙周组织中 TNF-α的表达。%Objective:To evaluate the effect of icariin on TNF-αexpression in periodental tissue during root resorption induced by ropid palatal expansion in rats.Methods:24 male Wistar rats were divided randomly into 3 groups(n =8)as group A(none-expansion control group),B(expansion group)and C(icariin and expansion group).In group B and C,an initial force of 50 g was applied to the area between the right and left upper first molars of the rats for 2 weeks for palatal expansion.The rats of group C were given intragastric administration of icariin at 2.5 mg/(kg·d)while the rats of group A and B were given the same dose place-bo.The centered buccal-lingual root and peridentium tissue slices were generated from the upper first molars of the rats.HE stai-ning of the slices was performed.The expression of TNF-αprotein at the compression sites was detected by irnmunohistochemistry. Results:The A values of TNF-αof group A,B and C were 0.030 2 ±0.001 7,0.241 0 ±0.002 4 and 0.088 6 ±0.002 8 respec-tively(B vs A or C,P <0.05).Conclusion:Icariin may inhibit TNF-αexpression in periodental tissue during root resorption in-duced by rapid palatal expansion.