Objective To investigate the effects of morphine combined with 5-fluouracil (5-Fu) on the expressions of Bcl-2 and Bax in MCF-7 breast cancer cells. Methods After being treated by morphine combined with 5-Fu for 48 h, the expressions of Bcl-2 and Bax in MCF-7 breast cancer cells were detected by Western Blot. Results The expression of Bcl-2 was the strongest in the untreated group, and was decreased by 250 μmol/L and 1 250 μmol/L of morphine and 500 fxmol/L of 5-Fu, and was decreased along with the increasing of morphine by the combination of morphine and 5-Fu. The expression of Bax was the weakest in the untreated group, and was increased by 250μmol/L and 1 250 μmol/L of morphine and 500 μmol/L of 5-Fu, and was increased along with the increasing of morphine by the combination of morphine and 5-Fu. The synergisms were found in all the 3 groups. Conclusions Morphine in certain concentration, 5-Fu and the combination of the two had the down-regulating effects on Bcl-2 expression and up-regulating effects on Bax expression in MCF-7 breast cancer cells, and in a certain dose dependent manner. Synergisms were shown in the combination of morphine and 5-Fu.%目的:研究吗啡与5-氟尿嘧啶(5-Fu)联合应用对MCF-7乳腺癌细胞Bcl-2、Bax表达的影响.方法:药物作用48 h后通过Western Blot检测Bcl-2、Bax表达情况.结果:Bcl-2表达情况:未处理组最强.单独应用吗啡时,250 μmol/L及1 250 μmol/L引起Bcl-2表达减弱.单独应用500 μmol/L 5-Fu时Bcl-2表达减弱.与5-Fu联合应用,随其中吗啡浓度的增加,Bcl-2表达逐渐减弱,且均具有协同作用.Bax表达情况:未处理组最弱.单独应用吗啡时,250μmol/L及1 250 μmol/L引起Bax表达增强.单独应用500 μmol/L 5-Fu时Bax表达增强.与5-Fu联合应用,随着吗啡浓度的增加,Bax表达逐渐增强,且均具有协同作用.结论:一定浓度的吗啡、5-Fu及联合应用于MCF-7乳腺癌细胞48 h能下调Bcl-2表达、上调Bax表达,且与其中吗啡的浓度有一定的剂量依赖关系,两药联合应用具有协同作用.
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