多重MSP检测早期鼻咽癌甲基化基因的初步探索

摘要

Objective To explore the effect of multiplex methylation specific PCR for early diagnosis of nasopharyngeal carcinoma. Methods The methylation status of 18 genes were verified by MSP from 35 early nasopharyngeal carcinoma andmultiplex-MSP was established through the optimized working condition for rapid detection of several genes′ methylation status. Results The methylation rate of 12 genes exceeds 50%. The methylation specific products in only one group (Segment U) did reach the expected results while those from the other three groups were consistent with the MSP′s. Conclusion Multiplex-MSP is an efficient and low-cost method, and it can produce ideal experimental results similar to those by the MSP and DNA microarrays.%目的：探讨多重MSP（multiplex-MSP）检测基因甲基化在鼻咽癌早期诊断中的作用。方法：通过MSP方法对35例Ⅰ期鼻咽癌标本18个基因的甲基化状态进行验证；同时通过条件摸索建立多重MSP方法，尝试实现多个基因甲基化状态的快速检测。结果：甲基化率超过50％的基因有12个；多重MSP检测中仅1组合的非甲基化特异性产物（U片段）扩增效果没有达到预期结果，余3个组合电泳结果与甲基化特异性PCR相符。结论：采取合适的组合进行多重MSP扩增可获得理想结果，其结果与MSP以及甲基化芯片扫描结果相似，是一种效率高，成本较低的实验方法。