白细胞介素24对急性白血病骨髓单个核细胞生长的影响

摘要

Objective The aim of this study was to observe the effects of interleukin-24 (IL-24) on the growth of acute leukemin bone marrow mononuclear cells (BMMNC) in vitro. And to investigate the mechanisms of IL-24 on acute leukemin BMMNC. Methods The group of acute lymphocytic leukemia (ALL) had 5 adults, the group of acute myeloid leukemia (ANLL) had 10 adults who did not receive any therapy. The acute leukemin BMMNC were separated from bone marrow. BMMNC were treated with IL-24 in vitro. The effects of IL-24 on acute leukemin BMMNC in vitro was detected by MTT assay. Apoptosis of acute leukemin BMMNC was measured by flow cytometry with AnnexinV-FITC staining. The expression of caspase-3 and bcl-2 in acute leukemin BMMNC was detected by RT-PCR. Results IL-24 can significantly inhibit the proliferation of acute leukemia BMMNC which was detected by MTT assay, and its effects were dose-dependent and time-dependent. After acute leukemia BMMNC were exposed to IL-24 for 48 h, the apoptotic rate of the group treated with IL-24 of 50 ng/mL was increased obviously. IL-24 increased the mRNA expression level of caspase-3, decreased the mRNA expression level of bcl-2 in acute leukemia BMMNC. Conclusion IL-24 can suppress the growth and induce apoptosis of acute leukemia BMMNC in vitro. IL-24 may inhibit the growth of acute leukemia BMMNC by up-regulating the expression of caspase-3 and down-regulating the expression of bcl-2.%目的：探讨白细胞介素24（IL-24）对成人急性白血病骨髓单个核细胞（BMMNC）体外生长的影响，并初步探讨其作用的可能机制。方法：随机选择在石河子大学一附院住院的急性白血病患者15例，均经FAB分型或免疫学分型确诊，其中确诊未治的成人急性淋巴细胞白血病（ALL）患者5例，成人急性非淋巴细胞白血病（ANLL）患者10例。取患者骨髓，分离单个核细胞，以不同浓度IL-24作用于成人急性白血病BMMNC。应用四氮唑蓝（MTT）比色还原法，检测IL-24对急性白血病BMMNC体外生长的影响；采用AnnexinV-FITC染色流式细胞术，观察检测IL-24对急性白血病BMMNC增殖状况的影响；利用逆转录-聚合酶链反应（RT-PCR），检测IL-24对急性白血病BMMNC中caspase-3和bcl-2表达的影响。结果：MTT法发现IL-24对体外培养的白血病BMMNC有明显的生长抑制作用，且呈时间、剂量依赖性，与阴性对照组相比差异有显著性（P＜0．05）。IL-24浓度50 ng/mL作用于急性白血病BMMNC 48 h后，Annexin V-PI检测结果显示凋亡率为（39．21±5．79）％，高于对照组（P＜0．01）。 RT-PCR检测显示，不同浓度IL-24处理白血病BMMNC，能够增加caspase-3 mRNA的表达，降低bcl-2 mRNA的表达。结论：IL-24对体外培养的成人急性白血病BMMNC，有明显的生长抑制、诱导凋亡作用。 IL-24可以上调caspase-3 mRNA表达，下调bcl-2 mRNA表达，可能是IL-24抑制急性白血病BMMNC生长作用的重要机制。