响应曲面优化紫花地丁总黄酮提取工艺研究

摘要

Optimization of extraction technology of flavonoids from Viola yedoensis Makino by Box-Behnken center composite design.Based on one-factor-at-a-time experiments,the quadratic regression model for the extraction efficiency of total flavonoids was established about the independent variables (ethanol volume fraction,ultrasonic power,the extracting temperature and the extracting time) through Box-Behnken center composite design and response surface methodology.The optimum extraction conditions were as follow:ethanol volume fraction was 64％,ultrasonic power was 161 W,the extracting temperature was 72 ℃,the extracting time was 32 min.Under these conditions,the practical yield of total flavonoids was 4.11％,which was consistent with the predicted yield of total flavonoids of 4.09％.Extraction model of total flavonoids from Viola yedoensis Makino established by Box-Behnken method can precisely predict the experiment results.In the best conditions deduced from the mode,the extraction rate of total flavonoids is high.%运用响应曲面设计(Box-Behnken设计)优化紫花地丁总黄酮提取工艺.在单因素试验基础上,选择乙醇体积分数、超声波功率、提取温度及提取时间为考查因素,采用响应曲面优化超声提取工艺条件,模拟得到总黄酮提取率二次回归方程预测模型.结果表明,最优提取工艺为乙醇体积分数64％、超声波功率161 W、提取温度72℃、提取时间32 min,总黄酮提取率实测结果(4.09％)与响应曲面拟合所得方程预测值(4.11％)符合良好.结果显示,采用Box-Behnken法建立紫花地丁总黄酮提取工艺模型得率高,并能很好地预测试验结果.