硅藻COX-Ⅰ区种属特异性PCR用于法医学溺死诊断

摘要

Objective:To develop and evaluate a method for identification of drowning death through detection of diatom COX-Ⅰ gene by primers pair of KEint2F and KEintR. Methods:Tissues of livers and lungs of twelve drowning cases and five non-drowning cases,field water from each case and double distilled water were collected,tested and compared two methods:diatom nitric acid diges-tion method and species specific PCR method,respectively. Results:The detection rate of diatom by PCR method was much higher than diatom nitric acid digestion method. Conclusion:KEint2F and KEintR are suitable primer pairs of species specific PCR for diatom de-tection for drowning identification.%目的：：对KEint2F和KEintR这一对COX-Ⅰ区特异性PCR引物应用于法医学溺死诊断的有效性进行研究。方法：取12例溺死尸体和5例非水中尸体的肺、肝组织，以及取落水区域水样及双蒸水，通过硝酸消化镜检法(镜检法)和种属特异性PCR检验法( PCR法)两种方法进行硅藻检验，对其硅藻的检出率进行对比。结果： PCR法比镜检法具有更高的硅藻检出率。结论： KEint2F和KEintR可作为法医学硅藻检验的特异性PCR引物用于法医学溺死的诊断。