依达拉奉对大鼠脊髓损伤过氧化物酶增殖物激活受体γ表达的影响

摘要

Objective: to study the adr in proliferation of rat spinal cord injury peroxide enzyme content activated receptor gamma(PPAR gamma)expression. Methods:10 weeks of age 60, SPF SD rats randomly divided into five groups:control group (the control group, 12), SCI + saline group (group, 12), SCI + in edaravone in group A (treatment group A, 12), the adr mentioned SCI + B group(treatment group B, 12), the adr mentioned SCI + C group (treatment group C, 12). Only do laminectomy, injury in rats control group and treatment group improved Allen 's method, make moderate animal models of spinal cord injury. Treatment group by 2 mg/kg, 3 mg/kg and 4 mg/kg dose of adr mentioned will use such as volume of 0.9% sodium chloride solution diluted, intraperitoneal injection once every 12 h, drug delivery time for 30 min, a total of 14 d. The control group and injury group methods give the same volume of 0.9%sodium chloride solution. Results: the treatment group, treatment group B, the treatment group C of PPAR gamma protein expression significantly increased in the control group, damage group (P<0.05), the treatment group, treatment group B, the treatment group C of PPAR gamma protein expression more significant difference (P<0.05). Conclusion:according to the adr can be by raising the expression of PPAR gamma to reduce inflammation, and with the increase of the dose, raise ability increases, which can effectively inhibit apoptosis around the spinal cord injury, significantly promote the cell metabolism, thus protecting the spinal cord, for the treatment of spinal cord injury and provide a new direction and new drug screening targets.%目的：观察依达拉奉对大鼠脊髓损伤过氧化物酶增殖物激活受体γ（PPARγ）表达的影响。方法：将10周龄SPF级SD大鼠60只，随机分为5组：假手术组（对照组，12只），SCI+生理盐水组（损伤组，12只），SCI+依达拉奉A组（治疗组A，12只），SCI+依达拉奉B组（治疗组B，12只），SCI+依达拉奉C组（治疗组C，12只）。对照组大鼠仅做椎板切除术，损伤组和治疗组采用改良Allen's法，制成中度脊髓损伤动物模型。治疗组按2mg/kg、3mg/kg和4mg/kg的剂量将依达拉奉用等体积的0．9％氯化钠溶液稀释后，每隔12h腹腔注射1次，给药时间为30min，共14d。对照组和损伤组采用同样方式给予等体积的0．9％氯化钠溶液。结果：治疗组A、治疗组B、治疗组C的PPARγ蛋白表达较对照组、损伤组明显增多（P＜0.05），治疗组A、治疗组B、治疗组C的PPARγ蛋白表达比较有显著差异（P＜0.05）。结论：依达拉奉可通过上调PPARγ的表达降低炎症反应，且随剂量增加上调能力增加，可有效抑制脊髓损伤周围细胞凋亡，显著促进细胞代谢，从而保护脊髓，为脊髓损伤的治疗及药物筛选提供新方向、新靶标。