响应面优化野艾蒿二咖啡酰奎尼酸的高效提取

摘要

采用响应面优化野艾蒿二咖啡酰奎尼酸的提取工艺.考察乙醇浓度、温度、时间、料液比4个重要因素对二咖啡酰奎尼酸得率的影响.在乙醇浓度72%、温度53℃、提取时间102 min、料液比1∶54( g/mL)条件下,二咖啡酰奎尼酸的提取率为91%,得率达3.30%；LC/MS等方法对野艾蒿中二咖啡酰奎尼酸的定量结果表明,野艾蒿中二咖啡酰奎尼酸由3,5-diCQA、4,5-diCQA和1,5-diCQA组成,其含量分别为14.2、10.5和8.3 mg/g,因而野艾蒿是一种二咖啡酰奎尼酸含量丰富的材料.%Response surface methodology is applied to optimize the extraction conditions of diCQAs from Artemisia lavan-dulaefolia DC. The combined effects of independent variables ( ethanol concentration, extraction temperature, extraction time,solid-liquid ratio) are studied and the optimal extraction conditions are as follows:ethanol concentration 72%, extraction temperature 53 ℃,extraction time 102 min,solid-liquid ratio 1∶54 g/mL. Under these conditions,the yield of diCQAs is 3.30% and the extraction yield is 91%. The content of diCQAs(3,5-diCQA,4,5-diCQA,1,5-diCQA) are 14.2 mg/g,10.5 mg/g and 8.3 mg/g respectively,which are identified by LC/MS. A. lavandulaefolia DC. is the materials that rich in dicaffeoylquinic acids.