转染IL－18基因的大鼠胶质瘤细胞疫苗9L／IL－18对胶质瘤大鼠中TGF－β和Fgf2水平的影响

摘要

目的：观察转染 IL －18基因的大鼠胶质瘤细胞疫苗9L／IL －18对胶质瘤大鼠血清 TGF －β和肿瘤组织中 Fgf2水平的影响。方法：将 F344大鼠随机分为3组，分别接种9L／IL －18、9L／LXSN 和9L 细胞。ELISA 法检测大鼠血清中 TGF －β水平，RT －PCR 方法检测肿瘤组织中 Fgf2 mRNA 的表达。结果：接种9L／IL －18细胞大鼠的外周血中 TGF －β的水平低于其他两组，差异有统计学意义（P ＜0．05），肿瘤组织中Fgf2 mRNA 的表达均低于其他两组（P ＜0．05）。结论：IL －18通过降低 TGF －β和Fgf2的水平，起到抗肿瘤的作用。%Objective:To assess the effect on the expression of TGF -βand Fgf2 of the exogenous IL -18 gene on the biological characteristics of rat 9L glioma cells.Methods:The cell suspension of 9L/IL -18,9L/LXSN and 9L cells were inoculated into the brain of F344 rat,detct the level of TGF -βand Fgf2 by enzymelinked immunosorbent assay and RT -PCR.Results:The level of TGF -βin the peripheral blood of rats inoculated 9L/IL -18 was weak-end to that inoculated 9L/LXSN and 9L(P <0.05).Compared to 9L/LXSN and 9L cells inoculated rat,the expression of Fgf2 were decreased in the tumor tissue of rat inoculated 9L/IL -18.Conclusion:IL -18 could decrease the levelof TGF -βand Fgf2.