IL-18对胶质瘤大鼠肿瘤组织中CD80、CD86及MHCⅠ类分子、Ⅱ类分子的影响

摘要

目的 观察转染IL-18基因的大鼠胶质瘤细胞疫苗9L/IL-18对大鼠胶质瘤肿瘤组织中CD80、CD86及MHCⅠ类分子、MHCⅡ类分子的影响,探讨IL-18在胶质瘤中的抗肿瘤作用.方法 F344大鼠随机分为3组,每组7只,分别接种9L/IL-18、9L/LXSN和9L细胞,流式细胞术检测CD80、CD86、MHCⅠ类分子、MHCⅡ类分子在肿瘤组织中的表达.结果 CD80、CD86、MHCⅠ类分子在接种9L/IL-18细胞组大鼠的肿瘤组织中的表达均明显高于接种9L/LXSN细胞组和接种9L细胞组大鼠的肿瘤组织中的表达,差异均有统计学意义(P<0殚.05);CD80、CD86、MHCⅠ类分子在接种9L/LXSN细胞组和接种9L细胞组大鼠的肿瘤组织中的表达,差异无统计学意义(P>0.05);MHCⅡ类分子在3组中的表达,差异无统计学意义(P>0.05).结论 IL-18通过促进肿瘤细胞表面CD80、CD86、MHCⅠ类分子的表达,促进树突状细胞分化成熟,进而激活T细胞,增强肿瘤细胞的抗原活性,提高免疫系统的监视功能,发挥抗肿瘤作用.%Objective To observe the effects of 9L/IL-18 on the expressions of CD80,CD86,MHCⅠand MHCⅡin tumour tissues of rats transfected with IL-18, and to explore the anti-tumour effects of IL-18.Methods The F344 rats were randomly divided into three groups ,with 7 rats in each group,and these rats were inoculated with 9L/IL-18 cells,9L/LXSN cells,9L cells,respectively.The expression levels of CD80,CD86,MHCⅠ,MHCⅡin tumor tissues were detected by flow cytometry.Results The expression levels of CD80,CD86,MHCⅠin tumor tissues of rats inoculated with 9L/IL-18 cells were significantly higher than those in tumor tissues of rats inoculated with 9L/LXSN cells and 9L cells ( P <0.05).However there were no significant differences in the expression levels of CD 80,CD86,MHCⅠbetween 9L/LXSN cell inoculation group and 9L cell inoculation group ( P >0.05).There were no significant differences in the expression levels of MHCⅡ among the three groups ( P >0 .05 ) .Conclusion IL-18 may promote the differentiation and maturation of dendritic cells by increasing the expressions of CD80, CD86, MHCⅠon the surface of tumor cells,thereby,which may activate T cells,enhance the antigent activity of tumour cells to improve the effects of immune surveillance and to produce anti -tumor effects .