吗啡联合厄洛替尼对人肺腺癌HCC827细胞增殖和凋亡的影响

摘要

目的:观察不同浓度吗啡联合厄洛替尼对HCC827细胞增殖活性及凋亡的影响.方法:不同浓度吗啡(250、500、1 000 μmol/L)和厄洛替尼(10、20、40μmol/L)处理HCC827细胞48 h后应用MTT法检测细胞增殖活性,流式细胞术检测对照组、吗啡、厄洛替尼单独处理以及吗啡联合厄洛替尼分别作用24 h后细胞凋亡率.结果:与对照组相比,不同浓度吗啡及厄洛替尼组细胞增殖活性均明显降低,吗啡、厄洛替尼及吗啡联合厄洛替尼组细胞凋亡率均增加.结论:大剂量吗啡可以协同厄洛替尼发挥抗肿瘤作用,其机制可能与诱导细胞凋亡相关.%Objective:To investigate the effect of Morphine combined with Erlotinib on the proliferation and apoptosis rate of HCC287 cell lines.Methods:MTT method was used to observe the proliferation rate at 48 h after the treatment of Morphine at different concentrations(250,500,1 000 μmol/L) and Erlotinib(10,20,40 μmol/L).Flow cytometry was used to detect the apoptosis rate after 24 h treatment in control,Morphine,Erlotinib and Morphine + Erlotinib group.Results:Compared to the control group,the proliferation rate of HCC827 cells was decreased in the Morphine and Erlotinib group,the apoptosis rate was increased in the Morphine,Erlotinib and Morphine + Erlotinib group.Conclusion:High-dose Morphine has a synergistic effect with Erlotinib hydrochloride in anti-tumor activity,which may be closely related to apoptosis.