攀枝花地区1~8岁健康儿童补体C3、C4含量检测及其参考范围的建立

摘要

目的建立攀枝花地区1~8岁儿童血清补体C3、C4含量的参考范围。方法应用美国贝克曼库尔特公司IMMAGE 800特种蛋白质分析仪，采用免疫速率比浊法对912例1~8岁健康儿童血清补体C3、C4含量进行检测，分成7个年龄段，对结果进行统计分析。结果攀枝花地区1~8岁健康儿童血清补体C3、C4含量在性别和各年龄段之间差别均无统计学意义（P>0.05），总的参考范围为C3：0.60~1.82g/L，C4：0.18~0.22g/L。同时与全国临床检验操作规程及厂家提供的参考范围相比较，差异均有统计学意义（P<0.05）。结论全国临床检验操作规程及厂家提供的参考范围已不适应本地区1~8岁儿童血清补体C3、C4的参考范围。各地各实验室应建立各自实验室的参考范围；若存在性别、年龄组间差异，应分别制定性别、年龄各段各自的参考范围。%Objective To establish the reference intervals of serum complement C3 ad C4 for 1 to 8 years old healthy children in Pan-zhihua area. Methods Immunoturbidimetry was applied in detecting serum complement C3 and C4 of 912 children of 1 to 8 years old with IMMAGE 800(Beckman-coulter company, USA)special protein analyzer;the results of 7 age stages were statistical analyzed. Re-sults The difference of sex and age in serum complement C3 and C4 among 1 to 8 years old healthy children in Panzhihua area was of no statistical significance(P>0.05), the reference intervals were as follows:C3:0.60~1.82g/L, C4:0.18~0.22g/L, which was of statisti-cal difference from those in“The National Clinical Test Regualation of Operation”and the reference intervals provided by the manufac-turers(P<0.05). Conclusions“The National Clinical Test Regulation of Operation”and the reference intervals provided by the manu-facturers are no longer suitable for the detection of serum complement C3 and C4 in 1 to 8 years old healthy children in Panzhihua area;each lab should set up its own reference intervals and reference intervals of sex and each age stage if there exists difference in sex and age.