1株近平滑假丝酵母的分离及其鉴定

摘要

A Candida parapsilosis (Cp) strain was isolated using YPD medium plate dilution method from soil and fruits, identified by molecular biological method with rRNA gene cloning and sequencing in its ITS transcription domain; and made the comparison with relevant sequence available in Genbank and phylogenetic analysis. The results of the sequencing showed that homologous rate of the Cp strain was 98.5％ - 100％ with the one in Genbank. Evolution analysis showed that it belonged to independent branch of Cp ( EF193067 ) and Cp UOA/HCPF ( FJ872013 ). Its morphology and molecular identification indicated that the Cp strain was successfully cultivated and this provided a foundation for its further development and utilization.%采用平板稀释法从土壤及水果中分离出1株近平滑假丝酵母(Candida parapsilosis),YPD培养基培养酵母,利用分子生物学方法对其rRNA基因内转录间区(ITS区)进行了克隆测序,并与GenBank中已有的有关序列进行比较及系统发育分析.测序结果表明该序列长度为547 bp,与GenBank中近平滑假丝酵母同源率在98.5%～100%之间,进化分析表明与C.parapsilosis(EF193067)、C.parapsilosis UOA/HCPF(FJ872013)属于一单独分支中,形态结果及分子鉴定表明近平滑假丝酵母培养成功,为其进一步开发利用提供了依据.