Tim-1分子在约氏疟原虫感染模型中的表达分析

摘要

T cell Ig and mucin 1 (TIM-1) molecular expression on the surface of T cells and regulatory B cells and was associated with the development of T helper (Th) 2 type immune response.In order to explore the role of Tim-1 molecule in the anti-malaria immune responses,the expression of Tim-1 and its relationship with the cytokine profile and the outcome of mouse malaria in lethal Plasmodium yoelii,P.yoelii infected mice were investigated.It was showed that BALB/c mice susceptible to P.yoelii and died at day 6 to day 7 post the infection,whereas DBA2 mice survived.The mRNA level of IFN-γin the spleen of BALB/c mice was significantly lower than DBA2 mice,while the IL-10 mRNA level was significantly higher than those of DBA2 mice at day 3 and day 5 post infection.The percentages of Tim-1 + T cells and Tim-1 + B cells in the spleen of BALB/c mice rose significantly at day 3 and day 5 post infection compared with uninfected mice,however,those of DBA2 mice barely changed before and after infection.The results suggested that Tim-1 involved in the regulation of immune response against P.yoelii and was likely related to the production of higher level of Th2 type cytokines in susceptible mice.%Tim-1分子表达在T细胞和调节性B细胞表面,具有促进Th2应答的作用.为探讨Tim-1分子在抗疟疾免疫应答中的作用,利用致死型约氏疟原虫(Plasmodium yoelii)感染鼠疟模型研究了Tim-1分子表达与小鼠感染结局和免疫应答模式的关系.结果显示,BALB/c小鼠对P.yoelii易感,在感染后6～7d全部死亡,感染后第3、5天脾内细胞因子IFN-γmRNA水平明显低于对P.yoelii抵抗的DBA2小鼠,而脾IL-10 mRNA水平显著高于DBA2小鼠.BALB/c小鼠脾内Tim-1+T、B细胞百分比在感染后第3、5天显著升高,而DBA2小鼠感染前后脾内Tim-1+T、B细胞百分比没有显著变化.结果提示,Tim-1分子参与抗P.yoelii免疫应答的调节,可能与易感鼠产生高水平Th2型细胞因子有关.