Distribution of Circumsporozoite Protein (CS) in Anopheles Stephensi MosquitoesInfected with Plasmodium Falciparum Malaria

摘要

Using immunohistochemistry that employed direct conjugation of alkalinephosphatase to a monoclonal antibody (PF 2A10) to the circumsporozoite protein of Plasmodium falciparum, the appearance of the CS protein was followed over time in the mosquito. The CS protein first appeared in vacuolated oocysts and as oocysts matured to the sporoblast, the amount of CS protein increased. CS protein was evident on the surface of sporozoites released into the hemolymph from ruptured oocysts between days 10-13 after the infective blood meal. Sporozoites did not remain very long in the hemolymph. Between 11-15 days after the infective blood meal, sporozoites appeared on the surfaces of the salivary glands depositing a significant amount of CS protein on the glands as they invaded. Small immunogenic pits were observed which mark the entrance of the parasite into the gland here was large variability in the labelling of parasites once they were in the glands. (jes)