碱性蛋白酶水解大豆分离蛋白制备大豆多肽的工艺条件优化

摘要

以大豆分离蛋白为原料，地衣芽胞杆菌产碱性蛋白酶水解大豆分离蛋白，分别从酶浓度、pH 值、反应温度和水解时间等因素来研究水解效果，在50℃，pH 10，酶浓度100 U/ mL，水解2 h 时水解效果最好，水解度达到31.45%。%Alkaline protease from Bacillus licheniformis was used to hydrolyze separated soybean protein as raw mate-rial,and the hydrolyses effects were studied respectively with enzyme concentration,pH,reaction temperature,hy-drolysis time,and other factors;under pH 10,50 ℃,reacted with 100 U/ mL enzyme concentration,hydrolyzing for 2 h the effect was the best with the degree of hydrolysis at 31. 45% .