影响马铃薯遗传转化效率的因素探讨

摘要

This research analyzed the factors affecting the transformation efficiency through transforming the genetic transformation of AtHKT1 gene into potato tetraploid cultivars via agrobacterium tumefacience mediated process.The research results showed:1.The efficiency of different explants was different ,the rooting rate of microtuber of all two potato cultivars were higher than that of minituber ,therefore microtu-ber was determined as the optimal transformation explants;2 .During the microtuber transformation the potato skin and eye should be removed;3 .The rooting rate could reach as high as 13 .3%when the diam-eter of microtuber was within the range of 0.5 cm and 1.0 cm;4.After the microtubers were stored under dark for 60 days at 4℃,the rooting rate can reach as high as 16 .0%.%通过农杆菌介导对马铃薯四倍体栽培种进行 AtHKT1基因的遗传转化，分析了影响转化效率的因素，结果表明：1．不同外植体的遗传转化的效率不同，两个品种的试管薯的抗性植株生根率均比微型薯的高，确定试管薯为最佳转化外植体材料；2．试管薯转化时应去除薯皮和芽眼；3．直径在0．5～1．0cm 之间的试管薯其抗性植株生根率最高，为13．3％；4．试管薯收获后在4℃，黑暗条件下贮藏60d时进行遗传转化，抗性植株生根率最高，为16．0％。