两亲梳状聚衣康酸的合成及其阿霉素的缓释体系

摘要

采用自由基聚合法合成聚衣康酸(PIA)，并将PIA接枝十二胺合成两亲性梳状聚合物(PIA-g-DDA).利用动态光散射(DLS)测试 PIA-g-DDA 的溶液性质，并研究其流体力学直径和烷基链接枝比率对粒径大小的影响.以芘为分子探针，通过荧光光谱法测定PIA-g-DDA的临界胶束浓度(CMC)；利用两亲性梳状聚合物 PIA-g-DDA 包埋阿霉素(DOX)，通过相转移的方法制备聚合物载药体系PIA-g-DDA@DOX.结果表明：烷基链接枝率越高，聚合物的胶束粒径越小；其CMC=2.01×10-2 mg/mL；该载药体系包埋率高，载药量大，缓释作用明显.%Poly(itaconic acid)was successfully synthesized by radical polymerization.Afterwards, comb-shape amphiphilic copolymers PIA-g-DDA were obtained via grafting docecylamine onto the backbone of PIA through amination reaction. Hydrodynamic diameter of the micelle formed by PIA-g-DDA in water was recorded by dynamic light scattering (DLS).The effect of graft density of alkyl on the size of the micelle was investigated,with the results indicating that an increase in the graft level of DDA leads to a decrease in the size of the micelle conversely. Critical micelle concentration (CMC)of the micelle was determined through fluorescence spectrophotometry with pyrene as probe to be 2.01×10-2 mg/mL.In the end,the copolymers were employed to encapsulate DOX (DOXorubicin)as a model,namely,drug delivery system PIA-g-DDA@DOX,to demonstrate an ideal encapsulation efficiency and drug loading value,and more importantly sustained release of drugs.