在pH 4.2～4.8的B-R缓冲介质中,莫西沙星(MXFX)和加替沙星(GTF)等氟喹诺酮类抗生素(FLQs)能与铜(Ⅱ)形成螯合阳离子,进一步与虎红(Tf)阴离子通过静电引力和疏水作用形成FLQs∶Cu(Ⅱ)∶Tf为1∶1∶1的离子缔合物,体系反应导致共振瑞利散射(RRS)显著增强并出现新的RRS光谱.两种药物的反应产物具有相似的光谱特征,最大RRS峰位于373 nm处,并在590 nm处有1个较小的散射峰.在373 nm处一定浓度的抗生素与散射增强(△I)成正比,MXFX和GTF的线性范围分别为0.031 ～7.8 mg/L和0.029～9.0 mg/L.据此建立了测定氟喹诺酮类药物的新方法,方法用于胶囊和人尿液中FLQs的测定并取得满意结果.同时对反应机理及RRS增强原因进行了讨论.%In pH 4. 2 -4. 8 Britton - Robinson( B - R) buffer solution, moxifloxacin( MXFX) and gatifloxacin(GTF) of fluoroquinolone antibiotics(FLQs) could react with Cu( II ) to form chelate cat-ions , which further bind with tetrachlorotetraiodo-fluoresceindisodium salt (Tf ) to form FLQs s Cu ( II ) : Tf ( 1:1:1) ion association complexes. The system reactions resulted in the great enhancement of resonance Rayleigh scattering( RRS) and new RRS spectra appeared. The reaction systems of two drugs had similar spectral characteristics, the maximum RRS wavelength situated at 373 nm, and one small scattering peak situated at 590 nm. The intensities of RRS at 373 run are proportional to the concentrations of antibiotics drugs in the ranges of 0. 031 - 7. 8 mg/L for MXFX and 0.029 -9.0 mg/L for GTF. Based on this, a new method was developed for the determination of FLQs in capsule and human urine samples. The reaction mechanism and the reasons for RRS enhancement of the system were also investigated.
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