A high performance liquid chromatography - electrospray ionization tandem mass spectro-metric( HPLC - ESI MS/MS) method was developed for the determination of seven plant growth regulator residues, including parachlorophenoxyacetic acid, gibberellic acid ( GA3 ) , 2, 4-dichlorophe-noxyacetic acid, α-naphthaleneacetic acid, indolebutyric acid, 6-benzylaminopurine and forehlor-fenuron in fruits and vegetables. Plant growth regulators were extracted twice from samples with meth-anol and the extracts were cleaned up on a Waters C18 solid phase extraction column(SPE, 0. 5 g, 3 mL). The qualitation of the analytes was achieved under selective reaction monitoring(SRM) mode, and the quantification of the analytes was carried out by the external standard method . The chromatography separation was performed on a Hypersil GOLD aQ (150 mm × 2. 1 mm, 3 μm) column by gradient elution with methanol and water as mobile phase. Under the optimal conditions, good linearities were obtained for seven plant growth regulators with their correlation coefficients no less than 0. 993 5. The average recoveries of seven plant regulators in fruits and vegetables samples at three spiked levels ranged from 79% to 97% , with relative standard deviations (RSDs) no more than 7.6% . The limits of detection (LODs) were in the range of 0.40 - 20.0 μg/kg. The result showed that the method was simple and sensitive, and was applicable for the detection of plant growth regulators.%建立了果蔬样品中对氯苯氧乙酸、赤霉酸( GA3)、2,4-二氯苯氧乙酸、α-萘乙酸、吲哚丁酸、6-苄氨基嘌呤、氯吡脲残留的高效液相色谱-串联质谱( HPLC - MS/MS)分析方法.样品用甲醇匀质提取2次,经Waters C18固相萃取小柱净化后,在高效液相色谱-串联质谱仪(HPLC - MS/MS)选择反应监测(SRM)模式下测定.采用质谱定性,外标法定量.色谱柱为Hypersil GOLD aQ(150 mm ×2.1 mm,3μm)柱,以甲醇和水为流动相,梯度洗脱.果蔬样品在低、中、高3个加标水平下的平均回收率为79％～97％,相对标准偏差均不高于7.6％.7种植物生长促进剂在果蔬样品中的方法检出限为0.40～20.0 μg/kg.该方法灵敏度高、操作简单,可作为大批量果蔬中植物生长促进剂残留的检测方法.
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