流动注射化学发光法测定利福喷丁

摘要

基于碱性介质中利福喷丁能增敏鲁米诺-铁氰化钾体系的化学发光这一现象,结合流动注射分析技术,建立了流动注射化学发光法测定利福喷丁的新方法.在最佳实验条件下,利福喷丁检测浓度的线性范围为0.008 ～0.88 mg/L,检出限(3σ)为1.2×10 3mg/L.对6.0×10-2 mg/L的利福喷丁进行平行测定,其相对标准偏差(RSD,n=l1)为1.9％.该方法已用于测定胶囊和血浆中利福喷丁的含量,结果满意.%A novel method of flow injection chemiluminescence for the determination of rifapentine was proposed based on the phenomenon that the chemiluminescence intensity of the luminol-K3Fe (CN) 6 system could be enhanced by rifapentine in alkaline medium,combined with flow injection analysis technique.Under the optimal experimental conditions,the linear range of rifapentine's detection concentration was 0.008-0.88 mg/L,the detection limit(3σ) was 1.2 × 10-3 mg/L,and the relative standard deviation(RSD,n =11) was 1.9％ for rifapentine at 6.0 × 10-2 mg/L.The method was applied in the determination of rifapentine in both capsules and plasma with satisfactory results.