A flow injection chemiluminescence method for the determination of protein using Copper(II)-Alizarin Red S complex as an efficient chemiluminescent probe

摘要

A sensitive flow injection chemiluminescence (CL) method is proposed for the determination of bovine serum albumin (BSA) using Copper(II)-Alizarin Red S (ARS) complex as an efficient chemiluminescent probe. The detection is based on the binding of the copper(II)- ARS complex to proteins and the catalytic activity of copper(II)-ARS in the luminol-H_2O_2 CL system. Under the selected conditions, the CL intensity is linear with the concentration of BSA in the range of 5.0 × 10~(-11) to 1.0 × 10~(-9) mol·L~(-1). The detection limit was 2.0 × 10~(-11) mol·L~(-1). The method is successfully applied to the determination of protein in urine.