液相色谱-三重四极杆质谱同时测定食品接触材料中双酚A、双酚F与双酚S的迁移量

摘要

采用液相色谱-三重四极杆质谱(LC-MS/MS)建立了食品接触材料中双酚A、双酚F和双酚S化合物的同时测定方法.双酚A、双酚F和双酚S在样品中通过与食品模拟物在一定温度、时间接触的状态下迁移到食品模拟物中,以C18色谱柱进行分离,甲醇-氨水为流动相洗脱,采用电喷雾离子源(ESI),多反应监测(MRM)负离子模式进行扫描,同位素内标法定量.结果表明,双酚A、双酚F和双酚S的色谱分离良好,双酚A和双酚F在1～50 μg/L质量浓度范围内与其峰面积均呈线性关系,BPS在0.1～ 50 μg/L质量浓度范围内与其峰面积呈线性关系.BPA和BPF的最低检出限为0.3μ.g/L,BPS的最低检出限为0.05 μg/L.加标回收率为83.6％ ～ 102.6％,相对标准偏差(RSD)为3.5％ ～ 8.9％.该方法快速可靠、准确简便,适用于食品接触材料中双酚A、双酚F和双酚S化合物的检测.%A high performance liquid chromatography-tandem mass spectarometry was established for the determination on migration amounts of bisphenol A,bisphenol F and bisphenol S in food contact materials.Bisphenol A,bisphenol F and bisphenol S were transfered into the food simulants in the sample contacted at a certain temperature and time.The separation was performed using a C18 chromatographic column with methanol-ammonia as mobile phase.Under the electrospray ionization (ESI) mode,bisphenol A,bisphenol F and bisphenol S were detected in a multiple-reaction monitoring mode(MRM),and quantified by the external standard method.The method indicated a perfect performance in chromatographic separation.The calibration curves of bisphenol A and bisphenol F were linear in the range of 1-50 μg/L,while the calibration curve of bisphenol S was linear in the range of 0.1-50 μg/L.The limits of detection were O.3 μg/L for bisphenol A and bisphenol F,and O.05 μg/L for bisphenol S.The recoveries ranged from 83.6％ to 102.6％,with relative standard deviations (RSDs) of 3.5％-8.9％.This method was rapid,accurate,precise and convenient,and was appropriate for the determination of bisphenol A,bisphenol F and bisphenol S in food contact materials.