内蒙古地区蒙古族ADAM33基因多态性与COPD易感性的研究

摘要

目的：了解内蒙古地区蒙古族人类解整连蛋白和金属蛋白( A disintegrin and metalloproteinase 33, ADAM33)基因单核苷酸多态性与慢性阻塞性肺疾病(COPD)的相关性。方法：采用引物序列特异性聚合酶链反应-限制性片段长度多态性( PCR-RFLP)方法,分别对内蒙古地区184名蒙古族COPD病人和203名蒙古族健康对照组的ADAM33基因的Q-1、T+1、T 2、T 1、S 1五个位点的单核苷酸多态性进行检测,观察两组之间基因型和等位基因频率的差异。结果：ADAM33基因的4个SNPs与COPD相关(Q-1,P<0.001；T+1,P=0.020；T 2,P=0.018；S 1,P<0.001)。结论：ADAM33基因的Q-1、T+1、T 2、S 1位点与内蒙古地区蒙古族COPD具有相关性。%Objective:To explore whether the SNPs in ADAM33 ( Q-1 , T+1 , T 2 , T 1 , S 1 ) was associated with COPD in population in Inner Mongolia. Methods:Genotyping was determined by using PCR-RFLP both in case and controls subjects. 184 COPD and 203 control subjects were selected. Results:We found that lower frequency of ADAM33 ( Q-1 , T+1 , T 2 , S 1 ) SNPs in COPD than in health control. Conclusions: Q-1、T+1、T 2、S 1 SNPs in ADAM33 were associated with COPD in Mongolian population in Inner Mongolia.