妊娠期高血压疾病患者胎盘中MMP-9、TIMP-1和EMMPRIN的表达及意义

摘要

Objective To study the expression and significance of matrix metalloproteinase (MMP)-9, tissue inhibitor of matrix metalloproteinase (TIMP) -1 and extracellular matrix metalloproteinase inducer (EMMPRIN) in placenta with hypertensive disorder complicating pregnancy. Methods Immunohistochemical method was used to detect the expression of MMP-9, TIMP-1 and EMMPRIN in placenta from 40 cases normal term pregnancy women and 55 cases of pregnancy women with hypertensive disorder complicating pregnancy (including 15 cases of pregnancy women with hypertension ,25 cases of pregnancy women with mild preeclampsia and 15 cases of pregnancy women with severe preeclampsia). Results The Expression of MMP-9 in placenta of preeclampsia was significantly lower than that in normal term pregnancy, which in severe preeclampsia group was significantly lower than in hypertension group, and in mild preeclampsia group was significantly lower than in the normal group (P<0. 05). Expression of TIMP-1 in placenta of preeclampsia was significantly higher than normal term pregnancy and hypertension group(P<0. 05). Expression of EMMPRIN in placenta of preeclampsia was significantly lower than that in normal term pregnancy, which in severe preeclampsia group was significantly lower than that in mild preeclampsia group and hypertension group(P<0. 05). Conclusion The lower expression of MMP-9 and EMMPRIN, and higher expression of TIMP-1 in placenta may contribute to the development of hypertensive disorder complicating pregnancy.%目的 研究妊娠期高血压疾病患者胎盘中基质金属蛋白酶(matrix metalloproteinase,MMP)9、基质金属蛋白酶组织抑制剂(tissue inhibitor of matrix metalloproteinase,TIMP)1以及细胞外基质金属蛋白酶诱导剂(extracellular matrix metalloproteinase inducer,EMMPRIN)的表达,探讨其与疾病的关系.方法 采用免疫组织化学SP法和二步法对40例正常足月妊娠妇女以及55例妊娠期高血压疾病患者(其中妊娠期高血压患者15例,轻度子痫前期患者25例,重度子痫前期患者15例)胎盘中MMP-9、TIMP-1、EMMPRIN的表达进行检测.结果 MMP-9的表达,轻度子痫前期组明显低于正常妊娠组,重度子痫前期组低于高血压组,轻度子痫前期组低于正常妊娠组,差异均有统计学意义(P＜0.05).TIMP-1的表达,子痫前期组高于正常妊娠组和高血压组,差异均有统计学意义(P＜0.05).EMMPRIN的表达,子痫前期组明显低于正常妊娠组,重度子痫前期组低于轻度子痫前期组及高血压组,差异均有统计学意义(P＜0.05).结论 妊娠期高血压疾病患者胎盘中滋养细胞MMP-9、EMMPRIN表达下降,TIMP-1表达升高,且其表达水平的高低可能与病情严重程度有关.