AB -8大孔树脂对芫荽黄酮的纯化工艺研究

摘要

利用AB-8大孔树脂纯化芫荽黄酮，通过静态和动态结合的方法，确定最佳工艺参数．结果表明，静态，吸附平衡时间为3 h，解析平衡时间为1．5 h；动态，上样液质量浓度为1．0 mg/mL，上样液pH值为6．0，上样流速为2 mL/min，洗脱剂质量浓度为70％乙醇溶液，洗脱流速为2 mL/min．在此条件下，AB-8大孔树脂可以较好的分离纯化芫荽中总黄酮．%AB-8 macroporous resin was used to separate and purify coriander flavonoids , static and dynamic adsorption and desorption behaviors of AB -8 macroporous resin were e-valuated for achieving the best purification of total flavonoids .The static:adsorption equilib-rium time was 4 h, desorption time was 1.5 h.The dynamic:the best adsorption was sample concentration of 1.0 mg/mL, pH of 6.0 and the flow rate of 2.0 mL/min.The desorption flow rate of 2.0 mL/min and 70% ethanol elution were the best .With definite technical process , the AB-8 macroporous resin could be used to purify the flavonoids of coriander .