子宫平滑肌中MAPK信号分子表达量与产后出血及BKCa、PGs表达的关系

摘要

[ABSTRACT]Objective:To study the relationship of mitogen-activated protein kinase (MAPK)signaling molecule expres-sion in uterine smooth muscle with postpartum hemorrhage as well as large-conductance calcium-activated potassium channels (BKCa)and prostaglandins (PGs)expression.Methods:Puerperae who received cesarean delivery in our hospital between May 2012 and December 2015 were selected,34 cases with postpartum hemorrhage caused by uterine inertia and 58 cases with nor-mal uterine contractions and without postpartum hemorrhage were screened and included in hemorrhage group and non-hemor-rhage group of the study respectively.During the cesarean delivery,proper amount of uterine muscle tissue was collected to de-termine uterine muscle contraction function as well as the content of MAPK signaling molecules,BKCa and PGs.ResuIts:The spontaneous in vitro uterine muscle contraction frequency,contraction amplitude and contraction activity as well as contraction frequency,contraction amplitude and contraction activity after oxytocin intervention of hemorrhage group were significantly lower than those of non-hemorrhage group;p-EKR1,p-ERK2,p-JNK1,p-JNK2,p-P38,PGE2 ,PGF2αand TXB2 content in uterine muscle of hemorrhage group were significantly lower than those of non-hemorrhage group while BKCaαsubunit andβsubunit mRNA content and protein content as well as 6-keto-PGF1αcontent were significantly higher than those of non-hemor-rhage group.p-EKR1,p-ERK2,p-JNK1,p-JNK2 and p-P38 content were positively correlated with PGE2 ,PGF2αand TXB2 , and negatively correlated with BKCaαsubunit andβsubunit as well as 6-keto-PGF1α.Conclusion:Lower expression of MAPK signal molecules in uterine smooth muscle will influence the expression of BKCa and PGs to lead to the occurrence of postpar-tum hemorrhage caused by uterine inertia.%目的：：研究子宫平滑肌中丝裂原激活蛋白激酶(MAPK)信号分子表达量与产后出血及大电导钙激活钾通道(BKCa)、前列腺素(PGs)表达的关系.方法：选择2012年5月~2015年12月在我院产科接受剖宫产的产妇,筛选发生宫缩乏力性产后出血的产妇34例和宫缩正常无产后出血的产妇58例,分别纳入研究的出血组和未出血组.剖宫产术中收集子宫肌组织适量,测定子宫肌的收缩功能以及MAPK信号分子、BKCa、PGs的含量.结果：出血组产妇离体子宫肌的自发性收缩频率、收缩幅度、收缩活动力以及缩宫素干预后收缩频率、收缩幅度、收缩活动力均显著低于未出血组(P＜0．05)；出血组产妇子宫肌中p-EKR1、p-ERK2、p-JNK1、p-JNK2、p-P38、PGE2、PGF2α、TXB2的含量显著低于未出血组(P＜0．05),BKCa通道α亚基和β亚基的 mRNA含量和蛋白含量以及6-keto-PGF1α的含量均显著高于未出血组(P＜0．05).p-EKR1、p-ERK2、p-JNK1、p-JNK2、p-P38的含量与PGE2、PGF2α、TXB2呈正相关,与BKCa通道α亚基和β亚基、6-keto-PGF1α呈负相关.结论：子宫平滑肌中MAPK信号分子表达下调会通过影响BKCa、PGs的表达来造成宫缩乏力性产后出血的发生.