非小细胞肺癌血浆中 miR-182的表达量及其与肿瘤 CT 表现、恶性生物学分子表达的关系

摘要

目的：：研究非小细胞肺癌血浆中 miR-182的表达量及其与肿瘤 CT 表现、恶性生物学分子表达的关系.方法：选择2012年5月~2015年10月期间在解放军第187医院诊断为非小细胞肺癌的60例患者纳入研究的 NSCLC 组，选择同期在我院体检且一般资料与 NSCLC 组患者配对的健康志愿者80例纳入研究的对照组.行增强 CT 检查并测量病灶的长径、短径，取血浆测定 miR-182的表达量以及CEA、CYFRA21-1、SCC-Ag、TSGF 的含量，取肿瘤组织并测定 RECK、MTSS1、PDCD4、DNMT3a 的含量.结果：NSCLC 组患者血浆中 miR-182的相对表达量显著高于对照组志愿者；血浆 miR-182高表达的非小细胞肺癌患者轴位最大长径、与其垂直的最大短径、冠状位最大垂直长径均显著高于血浆 miR-182低表达的非小细胞肺癌患者，血浆中 CEA、CYFRA21-1、SCC-Ag、TSGF 的含量显著高于血浆miR-182低表达的非小细胞肺癌患者，肿瘤组织中 RECK、MTSS1、PDCD4、DNMT3a 的含量显著低于血浆 miR-182低表达的非小细胞肺癌患者，两组间上述指标的差异均有统计学意义(均 P ＜0．05).结论：非小细胞肺癌血浆中 miR-182异常高表达且与肿瘤的 CT 表现、血浆肿瘤标志物含量以及肿瘤组织中恶性分子的表达密切相关.%Objective:To study the plasma miR-182 expression in non-small cell lung cancer and its relationship with tumor CT findings and malignant biological molecule expression.Methods:A total of 60 patients who were diagnosed with non-small cell lung cancer in our hospital from May 2012 to October 201 5 were included in NSCLC group of the study,and 80 cases of healthy volunteers who received physical examination in our hospital during the same period and whose general data matched with that of NSCLC patients were included in control group of the study.Contrast-enhanced CT was conducted and the major diameter and minor diameter of lesions were measured,plasma was collected to determine miR-182 expression as well as CEA, CYFRA21-1,SCC-Ag and TSGF levels,and tumor tissue was collected to determine the content of RECK,MTSS1,PDCD4 and DNMT3a.Results:Relative plasma miR-182 expression of NSCLC group was significantly higher than that of control group;axial maximum major diameter,maximum minor diameter perpendicular to it and coronary maximum vertical major di-ameter in non-small cell lung cancer patients with high plasma miR-182 expression were significantly higher than those in non-small cell lung cancer patients with low plasma miR-182 expression,plasma CEA,CYFRA21-1,SCC-Ag and TSGF levels were significantly higher than those in non-small cell lung cancer patients with low plasma miR-182 expression,RECK, MTSS1,PDCD4 and DNMT3a levels in tumor tissue were significantly lower than those in non-small cell lung cancer patients with low plasma miR-182 expression,and differences in above indexes between two groups were statistically significant (P <0.05).Conclusions:Plasma miR-182 expression is abnormally high in non-small cell lung cancer and is closely related to the CT findings of tumor,the content of serum tumor markers and the expression of malignant molecules in tumor tissue.