胃癌患者血清 HMGB 1及前哨淋巴结检测与肿瘤标志物含量、肿瘤组织恶性分子表达量的关系

摘要

Objective:To study the relationship of serum high mobility group box-1 protein (HMGB1 )and sentinel lymph node detection with tumor marker levels and malignant molecule expression levels in tumor tissue of gastric cancer pa-tients.Methods:Patients with early gastric cancer were selected as pathology group and healthy volunteers were selected as control group;serum HMGB1,carbohydrate antigen (CA)72-4,DDK1,thymidine kinase 1 (TK1 ),exosome,pepsinogen (PG)-Ⅰ and PG-Ⅱ levels were determined and PGR percentage was calculated;pathology group received intraoperative senti-nel lymph node localization and biopsy,tumor tissue was collected and the expression levels of malignant molecules were deter-mined.Results:Serum HMBG1,CA72-4,DDK1,TK1 and exosome levels of pathology group were significantly higher than those of control group (P <0.05 ),and PGR percentage was significantly lower than that of control group (P < 0.05 );the higher the serum HMBG1 level in gastric cancer patients,the higher the CA72-4,DDK1,TK1 and exosome levels and the lower the PGR percentage in serum;the higher the survivin protein levels,the lower the PTEN,p21,caspase-3 and caspase-7 levels in tumor tissue;CA72-4,DDK1,TK1 and exosome levels in serum and survivin protein level in tumor tissue of pa-tients with SLNS(+)gastric cancer were significantly higher than those of patients with SLNS(-)gastric cancer (P <0.05), and PGR percentage in serum and PTEN,p21,caspase-3 and caspase-7 protein levels in tumor tissue were significantly lower than those of patients with SLNS(-)gastric cancer (P <0.05).Conclusions:Serum HMGB1 and sentinel lymph node detection in gastric cancer patients can early assess tumor malignancy and lymph node metastasis.%目的：：研究胃癌患者血清中高迁徙率蛋白1(high mobility group box-1 protein,HMGB1)及前哨淋巴结检测与肿瘤标志物含量、肿瘤组织中恶性分子表达量的关系.方法：选择早期胃癌患者作为病例组,健康志愿者作为对照组,测定血清中 HMGB1、糖类抗原(CA)72-4、DDK1、胸苷激酶1(thymidine kinase 1,TK1)、核酸外切体(exosome)、胃蛋白酶原(PG)-I、PG-Ⅱ含量并计算 PGR 比例,病例组术中进行前哨淋巴结定位活检并收集肿瘤组织,测定恶性分子的表达量.结果：病例组血清中 HMBG1、CA72-4、DDK1、TK1、exosome 含量高于对照组(P ＜0．05),PGR 比例低于对照组(P ＜0．05)；胃癌患者血清中 HMBG1含量越高,血清中 CA72-4、DDK1、TK1、exosome 含量越高且 PGR 比例越低,肿瘤组织中 Survivin 的蛋白含量越高,PTEN、p21、Caspase-3、Caspase-7的蛋白含量越低；前哨淋巴结(SLN)(＋)胃癌患者血清中 CA72-4、DDK1、TK1、exosome 含量以及肿瘤组织中 Survivin 的蛋白含量显著高于 SLN(-)胃癌患者(P ＜0．05),血清中 PGR 比例以及肿瘤组织中 PTEN、p21、Caspase-3、Caspase-7的蛋白含量显著低于 SLN(-)胃癌患者(P ＜0．05).结论：胃癌患者血清中 HMGB1及前哨淋巴结检测能够早期评估肿瘤恶性程度及淋巴结转移.