白藜芦醇抑制小肠缺血再灌注继发肺损伤中P38MAPK通路及下游凋亡、炎症、氧化应激分子的表达

摘要

Objective: To study the protective effect of resveratrol on secondary lung injury induced by intestinal ischemia reperfusion and its effect on P38MAPK pathway.Methods: Adult male SPF SD rats were divided into control group, I/R group, Res-L group, Res-M group, Res-H group, the small intestine ischemia reperfusion model was made and Res-L group, Res-M group, Res-H group were given 5.0 mg/kg, 10.0 mg/kg, 15.0 mg/kg resveratrol.P38MAPK pathway molecules and downstream apoptotic molecules, inflammatory factors and oxidative stress products in the lung were determined.Results: P38MAPK, MAPKK, MAPKKK, Fas, FasL, Caspase-8, caspase-9, NF-kB, TNF-α, IL-1β expression and ROS, MDA contents in lung tissue of I/R group were significantly higher than those of the control group;P38MAPK, MAPKK, MAPKKK, Fas, FasL, Caspase-8, caspase-9, NF-kB, TNF-α, IL-1β expression and ROS, MDA contents in lung tissue of Res-L group, Res-M group and Res-H group were significantly lower than those of I/R group.Conclusions: resveratrol can inhibit apoptosis, inflammation and oxidative stress by inhibiting the function of P38MAPK pathway, and then protect the secondary lung injury induced by intestinal ischemia reperfusion.%目的:研究白藜芦醇对小肠缺血再灌注继发性肺损伤的保护作用及其对P38MAPK通路功能的影响.方法:选择成年雄性SPF级SD大鼠并分为对照组、I/R组、Res-L组、Res-M组、Res-H组,建立小肠缺血再灌注模型,Res-L组、Res-M组、Res-H组分别给予5.0 mg/kg、10.0 mg/kg、15.0 mg/kg白藜芦醇干预.检测肺脏中P38MAPK通路分子及下游凋亡分子、炎症因子、氧化应激产物的含量.结果:I/R组大鼠肺组织中P38MAPK、MAPKK、MAPKKK、Fas、FasL、caspase-8、caspase-9、NF-kB、TNF-α、IL-1β的表达量以及显ROS、MDA的含量显著高于对照组;Res-L组、Res-M组、Res-H组大鼠肺组织中P38MAPK、MAPKK、MAPKKK、Fas、FasL、caspase-8、caspase-9、NF-kB、TNF-α、IL-1β的表达量以及显ROS、MDA的含量著低于I/R组.结论:白藜芦醇能够通过抑制P38MAPK通路功能来减轻细胞凋亡、炎症反应和氧化应激反应,进而保护小肠缺血再灌注继发性肺损伤.