Resveratrol inhibits the P38MAPK pathway as well as downstream apoptosis, inflammation and oxidative stress molecule expression in secondary lung injury in intestinal ischemia-reperfusion

摘要

Objective: To study the protective effect of resveratrol on secondary lung injury in intestinalischemia reperfusion and its effect on P38MAPK pathway. Methods: Adult male SPF SDrats were selected and divided into control group, I/R group, Res-L group, Res-M group andRes-H group, the small intestinal ischemia reperfusion model was made, and Res-L group,Res-M group and Res-H group were given 5.0 mg/kg, 10.0 mg/kg and 15.0 mg/kg resveratrolfor intervention. The contents of P38MAPK pathway molecules as well as downstreamapoptotic molecules, inflammatory factors and oxidative stress products in the lung weredetected. Results: P38MAPK, MAPKK, MAPKKK, Fas, FasL, caspase-8, caspase-9, NF-kB,TNF-α and IL-1β expression as well as ROS and MDA contents in lung tissue of I/R groupwere significantly higher than those of control group; P38MAPK, MAPKK, MAPKKK, Fas,FasL, caspase-8, caspase-9, NF-kB, TNF-α and IL-1β expression as well as ROS and MDAcontents in lung tissue of Res-L group, Res-M group and Res-H group were significantlylower than those of I/R group. Conclusion: Resveratrol can inhibit the function of P38MAPKpathway to reduce apoptosis, inflammation and oxidative stress, and then protect the secondarylung injury induced by intestinal ischemia reperfusion.