肝癌患者血清ASPH、5'-NT含量与血管新生、癌细胞增殖的相关性

摘要

目的:研究肝癌患者血清ASPH、5''-NT含量与血管新生、癌细胞增殖的相关性.方法:选择在本院接受手术切除的原发性肝癌患者作为研究的肝癌组,同期在榆林市第三医院体检的健康志愿者作为研究的对照组.采集两组受试者的血清并检测ASPH、5''-NT及癌细胞增殖分子的含量;采集肝癌组患者的肝癌病灶、癌旁病灶并检测血管新生分子、癌细胞增殖分子的含量.结果:肝癌组血清中ASPH、5''-NT的含量均显著高于对照组;肝癌病灶中VEGF、VEGFR1、VEGFR2、HGF、EGFR、Bcl-2、Bcl-x、Bcl-w的含量均显著高于癌旁病灶且与肝癌患者血清中ASPH、5''-NT的含量呈正相关,肝癌病灶中Bax、Bak的含量显著低于癌旁病灶与肝癌患者血清中ASPH、5''-NT的含量呈负相关;肝癌组血清中AFP、GP73、GPC3、DKK1的含量显著高于对照组与肝癌患者血清中ASPH、5''-NT的含量呈正相关.结论:肝癌患者的血清ASPH、5''-NT含量显著升高且能准确评估血管新生及癌细胞增殖.%Objective: To study the correlation of serum ASPH and 5''-NT contents with angiogenesis and cancer cell proliferation in patients with liver cancer.Methods: Patients with primary liver cancer who underwent surgical resection in Yulin Third Hospital between December 2013 and December 2016 were selected as the liver cancer group of the research, and healthy volunteers who received physical examination in Yulin Third Hospital over the same period were selected as the control group of the research.Serum was collected from both groups to test the contents of ASPH and 5''-NT as well as liver cancer cell proliferation molecules;liver cancer lesions and para-carcinoma lesions were collected from liver cancer group to detect the contents of angiogenesis molecules and cancer cell proliferation molecules.Results:: Serum ASPH and 5''-NT contents in liver cancer group were significantly higher than those in control group;VEGF, VEGFR1, VEGFR2, HGF, EGFR, Bcl-2, Bcl-x and Bcl-w contents in liver cancer lesions were significantly higher than those in para-carcinoma lesions and positively correlated with serum ASPH and 5''-NT contents in patients with liver cancer while Bax and Bak contents in liver cancer lesions were significantly lower than those in para-carcinoma lesions and negatively correlated with serum ASPH and 5''-NT contents in patients with liver cancer;serum AFP, GP73, GPC3 and DKK1 contents in liver cancer group were significantly higher than those in control group and positively correlated with serum ASPH and 5''-NT contents in patients with liver cancer.Conclusions: Serum ASPH and 5''-NT contents increase significantly in patients with liver cancer and can accurately evaluate angiogenesis and cancer cell proliferation.